Next-generation sequencing for SARS-CoV-2 research
During viral pandemics, next-generation sequencing (NGS) can be a valuable tool for the characterization and detection of viruses in the environment, animals, and humans (Kustin et al. 2019). Additionally, NGS can provide useful insights to help researchers and clinicians looking to develop the right treatment options. We offer a variety of RNA-sequencing kits for unmatched sensitivity and reproducibility for all of your demanding sequencing applications, including research on SARS-CoV-2.
Monitoring of the environment
Monitoring environmental samples (e.g., tabletops, door handles, and other high touch points) for the presence of harmful pathogens requires a high degree of sensitivity, down to just a few copies of a viral or bacterial genome. Our SMARTer RNA-seq products offer sensitivity to detect trace amounts of RNA, and through randomly primed reverse transcription also enable detection of RNA that is in various stages of degradation. In particular, the SMARTer Pico v2 RNA-seq and SMART-Seq Stranded kits work from inputs of <1 ng total RNA to provide comprehensive RNA transcript profiling.
Pico v2 for coronavirus sequencing in the literature
Chen, L. and Zhong, L. Genomics functional analysis and drug screening of SARS-CoV-2. Genes & Diseases (2020). doi: https://doi.org/10.1016/j.gendis.2020.04.002
Lu, J. et al. Genomic Epidemiology of SARS-CoV-2 in Guangdong Province, China. Cell 181(5), 997–1003 (2020).
Pfefferle, S. et al. Complete Genome Sequence of a SARS-CoV-2 Strain Isolated in Northern Germany. Microbiology resource announcements 9(23), (2020).
Thair, S. et al. Transcriptomic Similarities and Differences in Host Response between SARS-CoV-2 and Other Viral Infections. medRxiv (2020). doi: https://doi.org/10.1101/2020.06.18.20131326
Wu, F. et al. A new coronavirus associated with human respiratory disease in China. Nature 579, 265–269 (2020).
Understanding the effects of viral infection
Our tools, such as the SMART-Seq v4 and SMART-Seq Single Cell kits, can help researchers understand the expression changes that occur in a host as a result of viral infection and treatment—at both the bulk and single-cell level, respectively—to aid in the development of appropriate treatment. Additionally, both of these kits sequence full-length transcripts enabling cell-type identification as well as providing detailed information on isoform changes, SNPs, or splice variants that can help us better understand the response to the infection and treatments alike.
SMART-Seq v4 for host-cell and SARS-CoV-2 mRNA expression in the literature
Kase, Y., Okano, H. Expression of ACE2 and a viral virulence-regulating factor CCN family member 1 in human iPSC-derived neural cells: implications for COVID-19-related CNS disorders. Inflamm Regener 40, 32 (2020).
Sharma, A. et al. Human iPSC-Derived Cardiomyocytes are Susceptible to SARS-CoV-2 Infection. Cell Rep. Med. 1, 100052 (2020).
For additional product information and a full listing of available sizes, please visit:
Kustin, T. et al. A method to identify respiratory virus infections in clinical samples using next-generation sequencing. Sci. Rep. 9, 1–8 (2019).
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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