Video protocol: editing human iPSCs with CRISPR/Cas9 and single-cell cloning
Our start-to-finish editing and single-cell cloning kits make human iPS cell editing routine instead of frustrating. In this video protocol, we provide step-by-step instructions for generating edited human iPS cell lines using our complete system for electroporation-based delivery of RNP complexes and single-cell cloning. This high-efficiency workflow greatly improves single-cell survival post-seeding, thanks to the Cellartis DEF-CS culture system. In addition to our electroporation-based kit, we also offer an alternate kit that uses gesicles to deliver RNP complexes.