T-cell receptor profiling
Cellular immunity is mediated by T cells, which participate directly in the detection and neutralization of pathogenic threats via T-cell receptors (TCRs). Given the relative specificity of TCR-antigen interactions, a high diversity of TCRs are required to recognize a myriad of pathogenic agents. To this end, the adaptive immune system has evolved a mechanism for somatic diversification of TCRs that is unrivaled in all of biology. TCRs are heterodimers composed of two distinct subunit chains, most commonly α and β chains, resulting from somatic rearrangements in a process called V(D)J recombination (Figure 1). An extensive repertoire of T cells with diverse TCR sequences is critical for the immune system to recognize mutated proteins and neoantigens from tumor cells. One challenge of immunogenomics applied to cancer is to determine T cell repertoires and to identify tumor-reactive T-cell clones, before and in response to immunotherapy in cancer patients (Liu et al. 2017).
SMARTer immune profiling kits leverage our SMART technology and a 5'-RACE-based approach to capture full-length information from V(D)J variable regions of TCRs. These kits streamline the process of sample preparation, provide reproducible results for a wide range of inputs of mouse/human samples (purified cells, spleen, PBMCs, Jurkat cells, etc.), and are highly sensitive in detecting low-abundance transcripts. SMARTer human/mouse TCR a/b profiling kits are the ideal tools for TCR profiling to gain insights into TCR repertoire diversity from bulk samples (total RNA or purified cells). Since the unique alpha-beta chain pairing of a TCR mediates antigen specificity, obtaining pairing information is crucial to gaining insights into antigen recognition, designing TCRs for targeted immunotherapy, and establishing ancestral relationships of T-cell populations (Wegehaupt et al. 2017).
The SMARTer Human scTCR a/b Profiling Kit enables the full capture of TCR-α and TCR-β variable regions to elucidate TCR α/β pairing information within single T-cells. Furthermore, high-throughput SMARTer human TCR a/b profiling is now available on the single-cell ICELL8 cx platform to capture complete V(D)J variable regions of TCR transcripts from >1,000 single cells.
Liu, X. S. & Mardis, E. R. Applications of immunogenomics to cancer. Cell 168, 600–612 (2017).
Wegehaupt, A. K. et al. Recovery and assessment of leukocytes from LR Express filters. Biologicals 49, 15–22 (2017).
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